Radioimmunoassay of cholecystokinin-pancreozymin
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چکیده
منابع مشابه
Radioimmunoassay of cholecystokinin-pancreozymin.
A sensitive and specific radioimmunoassay for cholecystokinin-pancreozymin (CCK-PZ) has been developed, using rabbit antisera to crude porcine hormone. Highly purified porcine CCK-PZ, labelled with (131)I, and repurified by column chromatography on Sephadex G15, was used as tracer. Separation of free from antibody-bound labelled CCK-PZ was carried out using charcoal, ion-exchange resin, or a do...
متن کاملEffect of secretin and cholecystokinin-pancreozymin
The inhibition of gastric motility induced by placing fat in the duodenum was demonstrated to be humoral by Farrell and Ivy (1926). Subsequent studies on extracts of duodenal mucosa have failed to define the substance involved, although the concept that 'enterogastrone' is a duodenal hormone inhibiting both gastric motility and acid secretion is accepted (Grossman, 1950). In recent years, speci...
متن کاملRadioimmunoassay of cholecystokinin in plasma.
A sensitive and specific radioimmunoassay for cholecystokinin (CCK) has been developed. Synthetic unsulfated carboxy-terminal fragment, CCK-8, was radioiodinated by the conventional Chloramine-T method. Antibodies were raised against sulfated CCK-8 covalently coupled to bovine thyroglobulin via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. By purification, highly immunoreactive 125I-labeled CC...
متن کاملRadioimmunoassay of cholecystokinin: production and evaluation of antibodies.
In order to produce antibodies for the radioimmunoassay of cholecystokinin the following animals were immunized: 10 rabbits and 3 guinea pigs with cholecystokinin 1—33 coupled to bovine serum albumin, 9 rabbits and 2 guinea pigs with uncoupled cholecystokinin 1-33, 5 rabbits with cholecystokinin 10-20 coupled to bovine serum albumin and 4 rabbits with sulphated cholecystokinin 26-33 coupled to ...
متن کاملCholecystokinin-converting enzymes in brain (cholecystokinin fragments/Michaelis constant/enzyme specificity/radioimmunoassay)
Crude extracts of porcine cerebral cortical tissue convert cholecystokinin (CCK) to its COOH-terminal fragments, the dodecapeptide (CCK-12) and the octapeptide (CCK-8). The Sephadex G-75 void volume eluate of the crude extract cleaves the arginine-isoleucine bond and effects conversion only to CCK-12; the Sephadex G-50 void volume eluate of the same extract cleaves the arginine-aspartate bond a...
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ژورنال
عنوان ژورنال: Gut
سال: 1974
ISSN: 0017-5749
DOI: 10.1136/gut.15.9.690